Activity of catechins and their applications

Background Catechins, which are polyphenol compounds found in many plants and are an important component of tea leaves, are strong anti-oxidants. Research Many studies seek to enhance the effects of catechins on the human body and boost their protective power against UV radiation. There are many examples of the positive anti-microbial, anti-viral, anti-inflammatory, anti-allergenic, and anti-cancer effects of catechins. Catechins increase the penetration and absorption of healthy functional foods and bio cosmetics into the body and the skin, thus improving their utility. High value-added anti-oxidant substances have been extracted from food and plant sludge, and experiments have shown that catechins are safe when applied to the human body. The stability of catechins is very important for their absorption into the human body and the effectiveness of their anti-oxidant properties. Conclusion Continued research on the strong anti-oxidant effects of catechins is expected to result in many advances in the food, cosmetics, and pharmaceutical industries.


Background
Catechins have many benefits including preventing or reducing skin damage. Catechins are important ingredients from tea leaves and have intensive anti-oxidant and representative physiological activities. They are members of the group of polyphenol compounds found in many medicinal plants. The major sources of catechins are Camellia sinensis (C. sinensis) and C. assumica. Green tea contains 75-80% water and polyphenol compounds (flavanols, flavandiols, flavonoid, and phenolic acid) (Zillich et al. 2015), and catechins account for more than 75% of the polyphenol compounds in tea leaves. They are condensation-type tannins with a ring and the basic structure of flavan-3-ol. They have many chemical structural features, such as hydroxyl groups (−OH), that combine easily with other materials (Singh et al. 2011). There are eight catechins (  (Jin et al. 2006), which are prominently present in green tea (Fung et al. 2012). Catechins provide several health advantages by scavenging free radicals and retarding extracellular matrix degradation induced by ultraviolet (UV) radiation and pollution (Shi et al. 2016). Catechins also directly affect the skin by activating collagen synthesis and inhibiting the production of matrix metalloproteinase enzymes (Arct et al. 2003). Because of the hydroxyl in the gallate group, EGCG and ECG are highly effective free-radical scavengers compared with many other standard anti-oxidants, such as ascorbic acid, tocopherol, and trolox (Gulati et al. 2009;Matsubara et al. 2013;Kim et al. 2018). Because of these useful actions, tea catechins are increasingly used in medical, pharmaceutical, and cosmetic products and are being actively studied in a variety of approaches.

Anti-oxidant activity
Catechins are well-studied substances with proven antioxidant effects. Studies have been conducted to boost the stability of catechins and increase their rate of absorption into the human body. Recent studies have focused on maximising the efficacy of anti-oxidants. Gallic acid and catechins show stable anti-oxidant activity by synthesis of galactan, and catechin anti-oxidants covalently bind to chains of proteins (Spizzirri et al. 2009).
Caesalpinia decapetala (C. decapetala) is effective in the oxidation stability of an oil-in-water emulsion (Gallego et al. 2017). Analysis using LC-ESI/LTQ Orbitrap/MS of autochthonous germplasm of the Campania region showed a higher level of anti-oxidant activity compared with the non-autochthonous germplasm (D'Urso et al. 2018). Enzymatic glucosylation of caffeic acid and EGCG leads to improved anti-oxidant ability in a cellular model of UV-induced skin ageing (Nadim et al. 2014). The flamboyant tree (Delonix regia) has potent anti-oxidant and anti-microbial activities (Feng et al. 2014). EGCG anti-oxidant capacity is effective against H 2 O 2 -induced human dermal fibroblast injury (Feng et al. 2013). Lipophilized EGCG derivatives show increased anti-oxidant activity (Zhong and Shahidi 2011). Flavonoids and triterpenoids from the fruit of Alphitonia neocaledonica have cytotoxicity, anti-oxidant, and anti-tyrosinase activities and are useful cosmetic ingredients (Muhammad et al. 2014). Approximately 106 phenolic compounds have been found using liquid chromatography assays coupled with electrospray ionisation for rapid profiling of phenolic compounds from red maple (Acer rubrum) leaves (Li and Seeram 2018). Bamboo stem extracts have demonstrated anti-melanogenic and anti-oxidative activities in a cell-free system and B16F10 melanoma cells (Choi et al. 2018). The ethanol extract of the marula tree is very effective in boosting activities in vitro. ECG and EGCG in marula tree extract contribute to anti-ageing activities (Shoko et al. 2018). Cocos nucifera bark showed anti-oxidant and anti-depressant activities through oxidative alterations in the prefrontal cortex (Lima et al. 2016).

UV protection activity
Extensive studies of the protective capacity of catechins against UV radiation have demonstrated that catechins are capable of enhancing the photo stability and protection of skin from UV rays. Studies have also been conducted to find effective uses for catechins in various fields, such as the prevention of skin ageing, by increasing their efficacy and stability. Catechins improve the stability of EGCG nanoethosomal suspensions to enhance the effectiveness of inhibiting UVB-induced skin damage (Zhang et al. 2016). Emulsification of catechins increases the permeation of the skin, protective capacity against UV rays, and anti-ageing effects (Yoshino et al. 2013). Various analyses, including3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and western blot assays, show that ECG is a powerful cure for UVB-induced damage to HaCaT keratinocytes (Huang et al. 2007). Exposure to simulated solar radiation with sunscreen sorbents showed that grape seed extracts have broad-spectrum protection due to their high photostability and a red shift over the entire UVA and UVB ray index (Martincigh and Ollengo 2016). Flavonoids show high light and heat stability in the preservation and release of methacrylic acid-grafted poly (N-vinyl-pyrrolidone) acid-grafted (N-vinyl-pyrrolidone) (Parisi et al. 2012). The inhibitory activity against mushroom tyrosinase of components isolated from Neolitsea aciculate demonstrates this plant could be a source of antimelanin-producing agents (Kim et al. 2012). Cultured UV-induced human keratinocytes were treated with EGCG, and the effects on inflammatory pathways and nuclear translocation of the transcription factor NF-κB were assessed. EGCG inhibited UVB-and UVA-induced inflammatory pathways and apoptosis in cultured human keratinocytes (Xia et al. 2005).

Anti-microbial activity
Research is underway to produce biological and functional cosmetics using the natural anti-microbial properties of catechins. Human epithelial KB cells cell experiments show thatflavan-3-ols and proanthocyanidin from Limonium brasiliense (L. brasiliense) interact with gingipains to inhibit the adhesion of Porphyromonas gingivalis (P. gingivalis) to epithelial host cells (de Oliveira et al. 2017). In studies of the anti-microbial activity of fullerene and its hydroxylated derivatives, C60 (OH)44 was as potent and broadly effective as catechin, which was used as a control for evaluation (Aoshima et al. 2009). Green tea extracts significantly reduced the levels of Streptococcus mutans (S. mutans) in saliva and dental plaques of children (Goyal et al. 2017).

Anti-allergenic and anti-inflammatory activities
Allergies are caused by an over active immune system reaction, producing itching and inflammation. Contact with certain allergens leads to a sensitive condition.
Studies have been conducted on the anti-allergenic activity of catechins. The anti-allergenic components of the oolong tea tree and the inhibitory activity of catechins on histamine released from rat peritoneal mast cells passively sensitised with the anti-egg albumin IgE antibody were investigated. GCG was the most potent anti-allergenic component among tea catechins (Ohmori et al. 1995). Extracts of Acerola bagasse (A. bagasse) can modulate the activity of proteases that act on coagulant, anti-coagulant, and thrombolytic activities as well as the destruction of phospholipids, thereby decreasing inflammation and platelet aggregation (Marques et al. 2018). Methanol extracts of the stem bark of Vitellaria paradoxa (V. paradoxa) showed anti-inflammatory and antiarthritic activities in acute and chronic inflammation in Wistar albino rats (Foyet et al. 2015). Chlorhexidine and green tea extracts reduced dentin corrosion and wear. Some matrix metallo protease inhibitors may be a preventative measure to prevent dentin erosion-abrasion (Magalhães et al. 2009).

Anti-viral and anti-cancer activities
Many studies have been conducted on the prevention and treatment of viral infections (measles, AIDS, chicken pox, SARS, MERS, Ebola, etc. ). An experimental study demonstrated the anti-influenza virus activity of green tea catechins (Ide et al. 2014). Ent EC-(4alpha→8) EC (Ent-Epiafzelechin-(4alpha -> 8) -epiafzelechin of Cassiajavanica did not affect cell viability and proliferation but interfered with herpes simplex virus cell penetration and adhesion (Cheng 2006). In clinical trials, gargling with green tea three times a day did not alter the rate of contracting the influenza virus. The researchers suggested that further study of catechin anti-viral activities are needed (Ide et al. 2014). Studies have found anticancer substances in plants that inhibit cancer cell proliferation, including catechins. Polyphenol-rich extracts from Lawsonia inermis (L. inermis) L. (Henna) inhibit oxidative radicals and cancer cell proliferation (Kumar et al. 2016).

Activation of skin barrier passage
Catechins have excellent anti-oxidant activity, but their high molecular weight and binding to the lipid bilayer of the skin are obstacles to passing the skin barrier. There have been numerous attempts to overcome this problem. Microneedle-mediated intradermal delivery enables EGCG to penetrate to deeper skin layers. Skin microporation with maltose microneedles facilitates the penetration of EGCG across the stratum corneum into the deeper skin layers, including the viable epidermis and dermis (Puri et al. 2016). Based on the use of oil-water emulsions with different oil contents, a mixture of polyphenols containing catechins using Franz-type diffusion cells permeated the epidermis and dermis in pig skin in vitro (Zillich et al. 2013). Hydrophilic additives reduce the activity of flavonoids by increasing their solubility. Skin penetration of flavonoids from grape leaf extract as well asrutin, quercetin, and catechins occurs through lipophilic membranes (Arct et al. 2002). EGCG, quercetin, 14-EGCG, and Ginkgo biloba extracts show excellent skin penetration in fresh white skin obtained from abdominal surgery on static Franz-type diffusion cells (dal Belo et al. 2009). Monoglycerol Ester (MGE)-liquid crystal (LC)-forming lipid and glycerol monoolate (GMO)-LC formulations have improved skin penetration from various physico-chemical properties of the drug. MGE formulations have lower viscosity, faster drug release, and better skin permeability than GMO formulations. The low viscosity of the MGE-LC-preparations might affect drug diffusion and permeability through the skin (Kadhum et al. 2017). Liposomes can actively pass skin layers through artificial phosphor lipid membranes.
Phospholipids have an outstanding affinity for certain groups of flavonoids, and a mixture of catechins and phytosomes, a complex of naturally active components and phospholipids (mainly lecithin), enhances skin elasticity (Bombardelli 1991). The interaction between fish collagen peptide (FCP) and EGCG was analysed using spectroscopic techniques, such as fluorescence spectres copy circular dichroism and Fourier-transform infrared spectroscopy (FTIR). More exposure of proline was found when FCP-EGCG complexes formed. FCP acts as an enhancer of EGCG and increases the absorption of EGCG into the skin and the body (Yang et al. 2015c). Chitosan microparticles containing green tea extracts show permeation of catechins into subcutaneous tissues, and metabolism studies show that chitosan microparticles improve subcutaneous delivery of catechins while limiting their degradation by skin enzymes (Wisuitiprot et al. 2011).

Promotion of cell activity
The effects of natural extracts, including catechins, on cell activity have been studied extensively. Extracts of black, green, and white tea have anti-melanogenic activities in immortalised melanocytes. Fermented tea leaves have the lowest cytotoxicity and the highest anti-melanogenic activities (Kim et al. 2015). EGCG reduced the secretion and production of melanin in human melanoma cells in a mechanistic study promoting skin hydration that measured anti-oxidant and pigmentation properties. EGCG increases hyaluronic acid synthase gene expression and cell proliferation ). EGCG-5′-O-α-glucopyranoside (EGCG-5′Glu), an EGCG derivative, has antioxidative effects in both cell-free and cellular systems. EGCG-5′Glu restores reactive oxygen species (ROS)-mediated cell viability, regulates caspases and cell survival molecules, and increases cell proliferation by modulating NF-κB activity .

Sludge utilisation
High value-added sludge utilisation in pharmaceuticals, cosmetics, and food has made considerable progress.
Tannin, an extract of solid waste produced in chestnut industrial processes, is an effective natural anti-oxidant for the cosmetic, food, and pharmaceutical industries (Aires et al. 2016). Used coffee grounds are a great source of bioactive compounds of interest to the cosmetic and pharmaceutical industries, and methylanthines and phenols are health-related compounds present in used coffee grounds. FTIR has been used to evaluate useful active ingredients in used coffee grounds (Magalhães et al. 2016). Identification and quantification of phenolic compounds and radical scavenging activities of the two by-products of Vitis vinifera L. cv noir showed that they have high potential as anti-oxidants (Reis et al. 2016). Procyanidins, composed of catechin oligomers, function in anti-oxidant activity, metal chelation, radical trapping, and direct enzyme binding. Based on these results, procyanidin oligomers strongly bind with permanent keratin hairs and inhibit hair destruction caused by oxidative damage (Kim 2011). Laccase catalyses the polymerisation of phenolic compounds, suggesting that laccase-catalysed polymerisation of natural phenols may be applied to the development of new cosmetic pigments (Jeon et al. 2010). In peel and seed tests, peel extracts show higher total polyphenol content and anti-oxidant activity (Kosińska et al. 2012). In characterisation and quantification analyses using HPLC-ESI-MS/MS, the highest anti-oxidant activity levels in the edible parts of araticum fruits were in the peel, followed by the pulp, and then the seeds (Arruda et al. 2017). In tests of cocoa bean husks using hot water treatment, anti-oxidant-rich extracts of phenol, sugar, and the obromine were produced when treated at 170°C for 30 min (Hernández-Hernández et al. 2018). Artocarpus heterophyllus (A. hererophyllus) shell is a good source of natural anti-oxidants and other physiologically active substances including catechins, according to the results of various analyses, such as LC-MS/MS and GC/ MS (Sharma et al. 2013). Sapucaia nuts and their byproducts are rich in phenolic compounds that are high in anti-oxidant activity. The content of phenol is especially high in the shell (Demoliner et al. 2018). In another study, the anti-oxidant activities of the bark fibres of four coconut cultivars were examined, and the phenolic components and anti-oxidant activities of the coconut shells were confirmed (Oliveira et al. 2013).

Stability
Catechins have high anti-oxidant activities and protect the skin from the sun's UV rays. Many studies are underway to stabilise catechins, which are highly unstable in sunlight. Addition of α-lipoic acid to catechins can produce an effective anti-oxidant by stabilising EGCG (Scalia et al. 2013). Components should be closely monitored when evaluating the compatibility of catechins and excipients commonly used for micro-and nanoemulsions in complementary and thermal assays. Especially for preparations containing liposomes, heat-based production processes should be avoided (Ferreira-Nunes et al. 2018). Flavonoids, alkaloids, and phenolic acids in green tea toothpastes were analysed for stability at various pH levels and were more stable at low pH (Jang et al. 2014). The photo stability of EGCG was examined under the same conditions using a water-soluble UVB filter, benzophenone-4(BP-4). The results showed that photo stability was concentration dependent; the maximum level of EGCG photo stabilisation (catechin loss, 29.4 ± 2.2%) was attained in the presence of 2.1% (w/w) BP-4 (Bianchi et al. 2011). A study of catechin-based collagen stabilisation showed that hydrophobic interactions and hydrogen bonding interaction affected collagen stabilisation by plant polyphenols (Madhan et al. 2005). C. decapetala extracts have anti-oxidant properties due to the phenolic compounds in the leaves. At a concentration of 0.2%, C. decapetala extracts reduced the oxidative degradation of the oil-in-water emulsion (Gallego et al. 2017).

Tissue biopsy culture model
The results of in vivo applications of catechins are not the same as in vitro results. Tissue bio culture models play a valuable role by replacing animal experiments in studies of catechins. To prove the principle that proteins and key gene markers may be altered in an optimised whole-tissue biopsy culture model, topical formulations containing green tea catechins were examined in a skin biopsy culture model (Sidgwick et al. 2016). EpiDerm has anti-oxidant properties like those of living organisms and can eliminate oxidative stress factors caused by EGCG under in vitro experimental conditions (Yuki et al. 2013). In an experiment using HaCaT and RBL-2H3 cells, the safety and anti-inflammatory effect of nanoencapsulated lipid-soluble green tea leaf extracts using the supercritical CO 2 extraction method were objectively proven (Shin et al. 2019).

Safety for human application
Although natural extracts are effective as anti-oxidants and anti-microbials, the safety of catechins should be ensured in actual human applications. Studies on the safety of catechins have been conducted in animal experiments and human clinical tests. Propionidin B-2 [EC-(4beta→8)-EC] promotes hair growth, and safety studies for human application are needed. Local procyanidin B-2 was safe and acceptable in a series of toxicity tests. Mutagenicity tests using guinea pigs, bacteria, and rabbits show that procyanidin B-2 is not a mutagen (Takahashi et al. 1999).
In characterisation and biocompatibility studies of "green" silver nanoparticles using green tea polyphenols, silver nanoparticles were nontoxic and biocompatible (Moulton et al. 2010).

Anti-oxidant properties of catechins used for other applications
In addition to their direct anti-oxidant activities, catechins are being studied to increase their utility in various fields. To increase the rate of the hair dying process using plant products, phenol-derived polymeric dyes from Trametes versicolor use a laccase reaction with catechins and catechol to achieve a permanent keratin hair dye of various colours and shades (Im and Jeon 2016). Food packaging materials or active membranes containing anti-oxidants, such as catechin-derived EGCG and EC, are a new way to reduce the oxidation of foods, cosmetics, and pharmaceuticals using biopolymer materials. The anti-oxidative activity of the film was measured by the removal of methanol extracts containing catechins and EC, and their amounts were 32.90% and 36.68%, respectively (Iñiguez-Franco et al. 2012). Tannic acid, EGCG, and ECG were bound to collagen by extensive hydrogen bonding augmented by hydrophobic interactions. They prevented the free access of collagenase to the active areas in collagen chains (Jackson et al. 2010).

Synergistic effects by the method and process of extraction
Many attempts have been made to improve the effectiveness and utilisation of catechins and to efficiently apply their anti-oxidant properties to the human body. Antioxidative and UV-barrier properties of the molecules can be used for cosmetic and dermatological formulations after a selective high-performance liquid chromatography (HPLC) method is developed and verified for evaluating the optimal efficacy of catechins in the development of topical formulations (Ferreira-Nunes et al. 2017). Near-infrared spectroscopy (NIRS) has been proposed as a rapid and non-destructive way to measure the contents of three main phenolics (caffeic acid, (+)-catechin, and chlorogenic acid) (Magalhães et al. 2016). Chemical modification of anthocyanin and procyanidins to more lipophilic compounds by mass spectroscopy has the advantage of increasing bioavailability in biological matrices because anti-oxidation activity increases based on the acylation of procyanidin B4 by saturated fatty acids (Cruz et al. 2015). Polyphenols and collagen peptides can be applied to the design of clear products, via the formation of lactoferrin (LF)-EGCG aggregates, which are destroyed chiefly through competition mechanisms with EGCG molecules (Yang et al. 2015a). The mechanism and structural properties of trivalent aggregates of LF and pectin in a multispectral analysis show that the fluorescence intensity of LF decreases while that of EGCG increases (Yang et al. 2015b). FTIR spectral analysis confirmed that the hydrogen bonds between the aliphatic, catechin, and aromatic hydroxyl groups on gelatin were responsible for the selfassembly of nanoparticles. In free-radical experiments, catechins could be protected by nanoparticles and last for an extended period (Chen et al. 2010). An efficient, precise, and reliable method was developed to quantify polyphenol catechins and EC in aguaraná extract solution using an HPLC-PDA method (Klein et al. 2012). Three different solvents and two extraction methods were used to compare the total polyphenol and flavonoid contents of tara pod extracts. The total polyphenol content was highest when a 75% ethanol solution was used in an hour-long ultrasonic process, and the flavonoid content was highest when it was extracted for 24 h in cold water. However, water extracts were effective only in the early stages of the oxidation process, showing that 75% ethanol extraction is the best method for polyphenol isolation (Skowyra et al. 2013). Asynergistic study between process parameters found that augmentation of the ultrasonic treatment process significantly speeds the recovery of phenolic anti-oxidants and reduces the processing time (Arruda et al. 2019). Procyanidin extracts of grape seeds prevent damage to most tissues and molecules from nanoparticle treatment (Niu et al. 2017). Table 1 summarises the activities of catechins and their applications. Catechins are used as materials to promote health, to prevent and treat diseases, and for cosmetic purposes. Studies of their high anti-oxidant activities found in plants and their by-products are continually being conducted. Extensive studies have been conducted on the UV protective activities of catechins to enhance their photo stability, efficacy, and stability for their use in various fields, including slowing the skin-aging process. EGCG nanoparticles inhibit UVA damage, grape seeds have photo stability against UVA rays, and catechins inhibit UVA-and UVB-induced inflammatory pathways. Anti-microbial activities of catechins were shown to inhibit the adhesion of P. gingivalis to host epithelial cells, as flavan-3-ols and proanthocyanidins of L. brasiliense interact with gingipains. Green tea extracts significantly reduce the levels of S. mutans in saliva as well as dental plaques. GCG is the most potent antiallergenic component among all tea catechins. Extracts of A. bagasse coagulants reduce inflammation and platelet aggregation. Extracts of V. paradoxa stem barks have anti-inflammatory and anti-arthritic properties. Green tea catechins have anti-influenza properties and have been shown to inhibit herpes simplex enzyme cell penetration and adhesion. Polyphenol-rich W-LI extracts from L. inermis (Henna) can inhibit oxidative radicals and cancer cell proliferation. Nanoparticles delivered through microneedles into human skin and oil-water emulsions with different oil contents enhance skin penetration and retention using Franz-type diffusion cells. The lipophilic membrane model increases rutin and quercetin contents, including flavonoids, and enhances skin penetration and retention due to the interaction of fish collagen and EGCG. FCP acts as an enhancer of catechins and increases absorption of catechins into the skin and the body, and chitosan microparticles improve the transdermal delivery of catechins. Catechin promotes cellular activities, and tea extracts inhibit melanin production. Fermented tea has the highest anti-melanogenic activity and the lowest cytotoxicity. Hyaluronic acid synthase reduces melanin secretion, EGCG derivatives restore ROS-mediated cell viability, and EGCG-5′Glu increases cell proliferation. Many studies have been conducted to obtain anti-oxidants from sludge, including chestnut shells, coffee grounds, A. heterophyllus shells, and coconut shells, with results that show good antioxidant activity. The use of sludge has been studied widely due to its high utility value, environmental protection, and interest in up-cycling products.

Conclusions
Catechins are highly unstable in sunlight, and research is underway to stabilise catechins. Addition of α-lipoic acid to catechins is effective for its stabilisation. BP-4, a soluble UVB filter, can stabilise EGCG to produce effective anti-oxidants. Catechin preparations made with lipid ingredients are less stable in heat, so it is necessary to  Gallego et al. 2017, Spizzirri et al. 2009, Nadim et al. 2014, Feng et al. 2014, Feng et al. 2013, Zhong and Shahidi 2011, Muhammad et al. 2014, Li and Seeram 2018, Choi et al. 2018, Shoko et al. 2018, Lima et al. 2016 UV protection Improve the stability of catechins in sunlight and increase the UV protection effect Zhang et al. 2016, Yoshino et al. 2013, Niu et al. 2017, Huang et al. 2007, Martincigh and Ollengo 2016, Parisi et al. 2012, Kim et al. 2012, Xia et al. 2005 Anti-microbial activities Demonstrates anti-microbial activity and develops pharmaceuticals and functional cosmetics de Oliveira et al. 2017, Aoshima et al. 2009, Goyal et al. 2017 Anti-allergenic and antiinflammatory activities Has an anti-allergenic component and antiinflammatory and anti-arthritic activity Ohmori et al. 1995, Marques et al. 2018, Foyet et al. 2015, Magalhães et al. 2009 Anti-viral activities Shows anti-influenza activity and interferes with cell infiltration and attachment of herpes simplex virus Ide et al. 2014, Cheng 2006 Anti-cancer activities Extract from Lawsonia inermis (Henna) can inhibit proliferation of cancer cells Kumar et al. 2016 Activation of skin barrier passage Various methods have enhanced the skin penetration of epigallocatechin-3-gallate (EGCG) anti-oxidants Puri et al. 2016, Zillich et al. 2013, dal Belo et al. 2009, Kadhum et al. 2017, Bombardelli 1991, Arct et al. 2002, Yang et al. 2015c, Wisuitiprot et al. 2011 Promoting cell activities Effective for low cytotoxicity and anti-melanin production and improves reactive oxygen species (ROS)-mediated cell viability and cell proliferation Kim et al. 2015 Sludge utilisation Anti-oxidant components were extracted from various sludge, suggesting that they could be developed into foods, cosmetics, and pharmaceuticals Aires et al. 2016, Magalhães et al. 2015, Reis et al. 2016, Kosińska et al. 2012, Arruda et al. 2017, Hernández-Hernández et al. 2018, Sharma et al. 2013, Demoliner et al. 2018, Oliveira et al. 2013 Stability Aspects exploited for improving stability include sunlight, oxidation, compound stability, and collagen stabilisation Scalia et al. 2013, Ferreira-Nunes et al. 2018, Jang et al. 2014, Bianchi et al. 2011, Madhan et al. 2005, Gallego et al. 2017 Relatively stable at low pH and the extraction efficiency of anti-oxidants was high Tsuchiya et al. 1997, Jang et al. 2014 Tissue biopsy culture model Demonstrated efficacy in vivo and vitro culture models Sidgwick et al. 2016, Ow and Stupans 2003, Yuki et al. 2013, Moulton et al. 2010 Safety for human applications In vivo experiments demonstrate safety Takahashi et al. 1999, Moulton et al. 2010 Anti-oxidant properties of catechins used for other applications Dyes, packaging materials, nanoparticles, and biocompatibility Im and Jeon 2016, Iñiguez-Franco et al. 2012, Kim 2011, Jeon et al. 2009, Rojas et al. 2005, Jackson et al. 2010 Synergistic effect by extraction method and process Chemical modification, molecular interaction mechanisms, hydrogen bonding, and nanoparticle treatment increase efficiency Ferreira-Nunes et al. 2017, Magalhães et al. 2016, Cruz et al. 2015, Yang et al. 2015a, Chen et al. 2010, Yang et al. 2015b, Niu et al. 2017, Klein et al. 2012, Skowyra et al. 2013, Arruda et al. 2019 avoid heating them as much as possible. The lower the pH level, the more stable the anti-oxidant activities of green tea compounds. This suggests that more research is required on the effect of pH on the various activities of catechins.
Catechins have been used in the tissue biopsy culture model to achieve optimised effects like those in an in vivo application. The anti-oxidant properties of Epi-Derm are like those of living organisms, and the stability and anti-inflammatory effects of catechins in HaCaT cells and RBL-2H3 cells were objectively proven. In safety tests for human applications, propionidinB-2 (epicatechin) was nontoxic and nonmutagenic. The antioxidant properties of catechins make them suitable for use in hair dyes and containers for medicines and cosmetics to reduce oxidation of the contents. HPLC, NIRS, nanoparticles, cold water, 75% ethanol solution, and ultrasonic treatment are proposed methods to increase human body applications and the extraction efficiency of catechins. All these studies and achievements suggest that the anti-oxidant activities of catechins will contribute significantly to the development of cosmetics and to human health.