Fig. 2

Effects of SME on melanogenesis and cell viability of B16 melanoma 4A5 cells. a Cellular melanogenesis of B16 melanoma 4A5 cells treated with SME. α-MSH-stimulated cells were treated with SME (0.3–10 μg/mL) or with 100 μM arbutin for 72 h and then lysed. Positive control cells were treated with 100 nM α-MSH and 0.1% DMSO alone, whereas negative control cells with 0.1% DMSO alone. The absorbance of cell lysates was measured at 415 nm. Values represent the mean ± SEM from three independent experiments. ^###p < 0.001 versus negative control, ^***p < 0.001 versus positive control by Tukey’s multiple comparison test. b Cell viability of B16 melanoma 4A5 cells treated with SME. α-MSH-stimulated cells were treated with SME (0.3–10 μg/mL) or with 0.1% DMSO alone for 72 h. Values represent the mean ± SEM from three independent experiments. NS indicates no statistical significance versus control by Dunnett’s multiple comparison test