Table 1 Primers used in Q-PCR
S. no. | Gene | Primer |
|---|---|---|
1 | IL-6 | Forward: 5′ -CCCTTCAGGAACAGCTATGAA- 3’ |
Reverse:5′ -ACAACATCAGTCCCAAGAAGG- 3’ | ||
2 | TNF-α | Forward: 5′ -GCCTCTTCTCATTCCTGCTC- 3’ |
Reverse: 5′ -GAGCCCATTTGGGAACTTCT- 3’ | ||
3 | iNOS | Forward: 5′ -CAGGTTGAGGATTACTTCTTCCA- 3’ |
Reverse: 5′ -TGTCAGAGTCTTGTGCCTTTG- 3’ | ||
4 | VEGF-A | Forward: 5′ -CGGAGAGCAACGTCACTATG- 3’ |
Reverse: 5 ‘-TGGTCTGCATTCACATCTGC- 3’ | ||
5 | TGF-β1 | Forward: 5′ -CCTGGAAAGGGCTCAACAC −3’ |
Reverse: 5′ -CAGTTCTTCTCTGTGGAGCTGA- 3’ | ||
6 | MMP-2 | Forward: 5′ -TTCTGTCCCGACCAAGGA- 3’ |
Reverse: 5′ -GGTGTAGATAGGGGCCATCA- 3’ | ||
7 | TIMP-2 | Forward: 5′ -CGTTTTGCAATGCAGACGTA- 3’ |
Reverse: 5′ -GATGGGGTTGCCATAGATGT- 3’ | ||
8 | Collagen-1 | Forward: 5′ -CAACAAGCATGTCTGGTTAGGA-3’ |
Reverse: 5′ -CACCCCTTCTGCGTTGTATT- 3’ | ||
9 | EGFR | Forward: 5′ -TCCATCCTAGAGAAAGGAGAGC-3’ |
Reverse: 5′ -GCGGCTATCAGCATCTATCAT-3’ | ||
10 | PGK1 | Forward: 5′ -GATGGGCTTGGACTGTGGTA- 3’ |
Reverse: 5′ -AACAGGACCGTTCCAAACAA- 3’ | ||
11 | PSMB2 | Forward: 5′ -GAGGGCAGTGGAGCTTCTTA- 3’ |
Reverse: 5′ -AGGTGGGCAGATTCAAGATG- 3’ | ||
12 | GAPDH | Forward: 5′-AATGTATCCGTTGTGGATCTGA - 3’ |
Reverse: 5′ - GCTTCACCACCTTCTTGATGT - 3’ |