Fig. 2
From: Hair growth-promoting effect of recombinant human sonic hedgehog proteins
Purification of the Shh protein with affinity chromatography. a SDS-PAGE analysis of the purification of Shh protein by Ni-affinity chromatography. Lane 1, lysate supernatant; lane 2, flow-through fraction; lane 3, molecular-mass marker; lanes 4–10, eluted with 20, 50, or 250 mM imidazole, respectively. b Purification of Shh by removing the His tag with thrombin. Lane 1, molecular-mass marker; lane 2, prior to digestion; lanes 3–8, after digestion for 2, 5, 10, 20, 30, and 40 min