Fig. 3

The effect of morin on GPx1 expression in UVA-irradiated HDFs. Quantitative real-time polymerase chain reaction (qRT-PCR) was used to quantitatively analyze gene expression associated with morin in HDFs. qRT-PCR is a method for quantitatively measuring the level of DNA and the degree of activity of a specific gene. The amount of gene expression was measured using SYBR green in the double strand of extracted mRNA, and the validity of the PCR was confirmed by a melting curve. HDFs (2 × 105) were seeded in a 60-mm culture dish and incubated for 24 h. Prior to UVA exposure, the cells were pretreated with various concentrations of morin. Then, the cells were washed with PBS and irradiated by 10 J/cm2 UVA. After further incubation for 24 h, the expression level of GPx1 mRNA was measured by qRT-PCR. The data are expressed as mean ± SD of the relative cell viability in each sample from triplicate experiments (^##p < 0.01, **p < 0.01, ***p < 0.001)