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Fig. 7 | Biomedical Dermatology

Fig. 7

From: Antiapoptotic effects of scutellarin on ultraviolet A-irradiated HaCaT human keratinocytes

Fig. 7

Effect of scutellarin on tailed DNA in UVA-induced HaCaT cells. Comet assay reagent kits (Trevigen, USA) were used. After completely dissolving LMAgarose for 5 min, the cells at a concentration of 1 × 105 and LMAgarose were mixed in a 1:10 (v/v) ratio. Then, 50 μL of the mixture was placed on the Comet slide. With the slides in an alkaline electrophoresis solution, the cells were electrophoresed at 21 V for 30 min. They were dyed for 30 min with the dried agarose gel in SYBR Gold. Then, the fully dried 37 °C agarose gel was observed using a fluorescence microscope. The graph represents the mean ± SD of the relative expression level of tailed DNA in each sample from triplicate experiments. (**p < .01, ###p < .001)

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