Fig. 6

Effect of scutellarin on △Ψm in UVA-induced HaCaT cells. JC-1 (5,5′,6,6′-tetrachloro-1,1′,3,3′-tetraethyl-benzimidazolylcarbocyanine iodide) mitochondrial membrane potential assay kit (Cayman chemical, USA) was used. HaCaT cells were cultured for 24 h after cell seeding and re-cultured for another 24 h after sample and stimulus processing to the cells. After adding JC-1 for reaction for 20 min in 37 °C, it was washed twice with DPBS buffer (100 μl/ml of culture medium). Each florescence was measured by a flow cytometer (red: excitation 550 nm, emission 600 nm/green: excitation 485 nm, emission 535 nm). The red/green fluorescence ratio was measured and indicated in mitochondrial depolarization (**p < .01, ***p < .001, ###p < .001)