Fig. 3

Effect of scutellarin on UVA-induced lipid peroxidation in HaCaT cells. The lipid peroxidation analysis was examined by the lipid peroxidation (MDA) assay. Prior to UVA, the cells were pre-treated with various concentrations of scutellarin. HaCaT cells were homogenized on ice with the MDA lysis buffer and centrifuged (13,000×g, 10 min) to separate the supernatant (200 μl). The TBA solution (600 μl) was added to each test tube for 1 h at 95 °C, and 10 min on ice, and dispensed in a 96-well by 200 μl for analysis. A microplate reader (Bio-Tek, USA) was used to measure in 586 nm. (**p < .01, ***p < .001, ###p < .001)