Table 1 The primers used in qRT-PCR
From: Sophora japonica extracts accelerates keratinocyte differentiation through miR-181a
Gene | Primer sequence |
|---|---|
K1 | F: 5′-CAGCATCATTGCTGAGGTCAAGG-3′ R: 5′-CATGTCTGCCAGCAGTGATCTG-3′ |
K10 | F: 5′-TGGTTCAATGAAAAGAGCAAGGA-3′ R: 5′-GGGATTGTTTCAAGGCCAGTT-3′ |
Involucrin | F: 5′-CAAAGAACCTGGAGCAGGAG-3′ R: 5′-CAGGGCTGGTTGAATGTCTT-3′ |
Filaggrin | F: 5′-GCAAGGTCAAGTCCAGGAGAA-3′ R: 5′-CCCTCGGTTTCCACTGTCTC-3′ |
β: 5′-C | F: 5′-GGATTCCTATGTGGGCGACGA-3′ R: 5′-CGCTCGGTGAGGATCTTCATG-3′ |