Fig. 1

Screening of methanolic extracts from leaves of 47 plant species for inhibitory activity on melanogenesis. B16 melanoma 4A5 cells stimulated with 100 nM α-MSH were treated with 10 μg/mL of each methanolic extract or with 0.1% DMSO alone (control) for 72 h. Then, the cells were lysed and the absorbance was measured at 415 nm. Relative melanin content (%) was shown as the melanin content of control cells to be 100%. Data are shown as the mean ± SEM (n = 2). ^*p < 0.05 versus control by Dunnett’s multiple comparison test