Fig. 1
From: Hair growth-promoting effect of recombinant human sonic hedgehog proteins
Expression of Shh protein in E. coli BL21(DE3)pLysS by making silent mutations in 12 amino acids from N-terminus of Shh. a SDS-PAGE analysis of the expression of Shh in which the 20 amino acids in the 5′ region randomly changed. Lane M, molecular-mass marker; lane mock, uninduced; lane wt, no DNA mutations of Shh; lanes #3, #5, #6, #10, #15, #16, #20, #22, and #23, randomly changed colonies. b DNA sequence of colony #6, #10, #16 and #22 were shown